Cold‐stored leukoreduced whole blood: Extending the time between donation and filtration has minimal impact on in vitro quality

Background Leukoreduced whole blood (LR-WB) has received renewed attention as alternative to component-based transfusion in trauma. According the manufacturer's instructions, leukoreduction should be carried out within 8 h after collection. This study assessed impact of (1) WB collection bag, (2) LR filtration, and (3) timing filtration on vitro quality. Study Design Methods collected into different vendor bags was held at room temperature for <8 or >16 but <24 prior LR. In quality before throughout 3 weeks storage 4°C. Cell count hemoglobin levels were determined by hematology analyzer, platelet activation, responsiveness ADP surface expression P-selectin flow cytometry, hemolysis HemoCue, metabolic parameters gas analyzer. Hemostatic properties rotational thromboelastometry. Plasma protein activities clotting times automated coagulation analyzer quantitative immunoblotting. Results Bag type had no some impact, is aligned with data literature. The time between donation resulted statistically significant differences activity, yield, factor activity initially; however, these attributes decreased 21-day cold storage. Conclusion hold showed only a minor quality, so it may possible processing facilities explore extended order provide greater operational flexibility.